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9787030386069：分子克隆实验指南（第四版）

作者: MICHAEL 著 , JOSEPH SAMBROOK 著

出版社: 科学出版社

出版时间: 2013-01

版次: 1

ISBN: 9787030386069

定价: 2200.00

装帧: 平装

开本: 16开

纸张: 胶版纸

页数: 2028页

字数: 3千字

正文语种: 英语

原版书名: Molecular Cloning: A Laboratory Manual (Fourth Edition)

丛书: Molecular Cloning: A Laboratory Manual(Fourth Edition)

分类: 自然科学

2人买过

　　Molecular Cloning has served as the foundation of technicalexpertise in labs worldwide for 30 years. No other manual has beenso popular， or so influential. Molecular Cloning， Fourth Edition，by the celebrated founding author Joe Sambrook and new co-author，the distinguished HHMI investigator Michael Green， preserves thehighly praised detail and clarity of previous editions and includesspecific chapters and protocols commissioned for the book fromexpert practitioners at Yale， U Mass， Rockefeller University， TexasTech， Cold Spring Harbor Laboratory， Washington University， andother leading institutions. The theoretical and historicalunderpinnings of techniques are prominent features of thepresentation throughout， information that does much to helptrouble-shoot experimental problems.
　　For the fourth edition of this classic work， the content hasbeen entirely recast to include nucleic-acid based methods selectedas the most widely used and valuable in molecular and cellularbiology laboratories.
　　Core chapters from the third edition have been revised tofeature current strategies and approaches to the preparation andcloning of nucleic acids， gene transfer， and expression analysis.They are augmented by 12 new chapters which show how DNA， RNA， andproteins should be prepared， evaluated， and manipulated， and howdata generation and analysis can be handled.
　　The new content includes methods for studying interactionsbetween cellular components， such as microarrays， next-generationsequencing technologies， RNA interference， and epigenetic analysisusing DNA methylation techniques and chromatin immunoprecipitation.To make sense of the wealth of data produced by these techniques， abioinformatics chapter describes the use of analytical tools forcomparing sequences of genes and proteins and identifying commonexpression patterns among sets of genes.
　　Building on thirty years of trust， reliability， and authority，the fourth edition of Molecular Cloning is the new goldstandard—the one indispensable molecular biology laboratory manualand reference source. 　　JOSEPH SAMBROOK：

　　Sambrook was educated at the University of Liverpool (BSc (hons)

1962) and obtained his PhD at the Australian National University in

1966. He did postdoctoral research at the MRC Laboratory of

Molecular Biology (1966-67) and the Salk Institute for Biological

Studies (1967-69). In 1969 he was hired by James D. Watson to work

at the Cold Spring Harbor Laboratory in New York. Watson has been

reported to say this was the best hiring decision he ever made. Joe

was responsible for creating a combative creative environment at

CSHL that fomented discovery. Subsequently he worked at the

University of Texas Southwestern Medical Center (Dallas).

Sambrook is best known for his studies on DNA tumor viruses and

the molecular biology of normal and neoplastic cells. His Tumour

Virus Group at Cold Spring Harbor identified and mapped all of the

major genes of adenoviruses and SV40, determined their

transcriptional control in infected and transformed cells, and

elucidated the mechanism of integration of these viruses into the

genome of the host cell.[citation needed] He has also made

important contributions to the understanding of intracellular

traffic and protein folding and is an influential leader in the

field of the molecular genetics of human cancer. VOLUME1
Part1：Essentials
1.IsolationandQuantificationofDNA
2.AnalysisofDNA
3.CloningandTransformationwithPlasmidVectors
4.GatewayRecombinationalCloning
JohnS.Reece-HoyesandAlberthaJ.M.Walhout
5.WorkingwithBacterialArtificialChromosomesandOtherHigh-CapacityVectors
NathanielHeintzandShiaochingGong
6.Extraction，Purification，andAnalysisofRNAfromEukaryoticCells
7.PolymeraseChainReaction
8.Bioinformatics
Jui-HungHungandZhipingWeng

VOLUME2
Part2：AnalysisandManipulationofDNAandRNA
9.QuantificationofDNAandRNAbyReal-TimePolymeraseChainReaction
10.NucleicAcidPlatformTechnologies
OliverRando
11.DNASequencing
ElaineMardisandW.RichardMcCombie
12.AnalysisofDNAMethylationinMammalianCells
PaulM.Lizardi，QinYan，andNarendraWajapeyee
13.PreparationofLabeledDNA，RNA，andOligonucleotideProbes
14.MethodsforInVitroMutagenesis
MatteoForloni，AlexLiu，andNarendraWajapeyee

Part3：IntroducingGenesintoCells
15.IntroducingGenesintoCulturedMammalianCells
PritiKumar，ArvindhanNagarajan，andPradeepD.Uchil
16.IntroducingGenesintoMammalianCells：ViralVectors
GuangpingGaoandMiguelSena-Esteves

VOLUME3
Part4：GeneExpression
17.AnalysisofGeneRegulationUsingReporterSystems
PradeepD.Uchil，ArvindhanNagarajan，andPritiKumar
18.RNAInterferenceandSmallRNAAnalysis
ChengjianLiandPhillipD.Zamore
19.ExpressingClonedGenesforProteinProduction，Purification，andAnalysis
ClaraL.Kielkopf，WilliamBauer，andInaUrbatsch

Part5：InteractionAnalysis
20.Cross-LinkingTechnologiesforAnalysisofChromatinStructureandFunction
TaeHoonKimandJobDekker
21.MappingofInVivoRNA-BindingSitesbyUV-Cross-LinkingImmunoprecipitation（CLIP）
JenniferC.Darnell，AldoMele，KaYingSharonHung，andRobertB.Darnell
22.Gateway-CompatibleYeastOne-HybridandTwo-HybridAssays
JohnS.Reece-HoyesandAlberthaJ.M.Walhout

Appendices
1.ReagentsandBuffers
2.CommonlyUsedTechniques
3.DetectionSystems
4.GeneralSafetyandHazardousMaterial
内容简介:
　　Molecular Cloning has served as the foundation of technicalexpertise in labs worldwide for 30 years. No other manual has beenso popular， or so influential. Molecular Cloning， Fourth Edition，by the celebrated founding author Joe Sambrook and new co-author，the distinguished HHMI investigator Michael Green， preserves thehighly praised detail and clarity of previous editions and includesspecific chapters and protocols commissioned for the book fromexpert practitioners at Yale， U Mass， Rockefeller University， TexasTech， Cold Spring Harbor Laboratory， Washington University， andother leading institutions. The theoretical and historicalunderpinnings of techniques are prominent features of thepresentation throughout， information that does much to helptrouble-shoot experimental problems.
　　For the fourth edition of this classic work， the content hasbeen entirely recast to include nucleic-acid based methods selectedas the most widely used and valuable in molecular and cellularbiology laboratories.
　　Core chapters from the third edition have been revised tofeature current strategies and approaches to the preparation andcloning of nucleic acids， gene transfer， and expression analysis.They are augmented by 12 new chapters which show how DNA， RNA， andproteins should be prepared， evaluated， and manipulated， and howdata generation and analysis can be handled.
　　The new content includes methods for studying interactionsbetween cellular components， such as microarrays， next-generationsequencing technologies， RNA interference， and epigenetic analysisusing DNA methylation techniques and chromatin immunoprecipitation.To make sense of the wealth of data produced by these techniques， abioinformatics chapter describes the use of analytical tools forcomparing sequences of genes and proteins and identifying commonexpression patterns among sets of genes.
　　Building on thirty years of trust， reliability， and authority，the fourth edition of Molecular Cloning is the new goldstandard—the one indispensable molecular biology laboratory manualand reference source.
作者简介:
　　JOSEPH SAMBROOK：

　　Sambrook was educated at the University of Liverpool (BSc (hons)

1962) and obtained his PhD at the Australian National University in

1966. He did postdoctoral research at the MRC Laboratory of

Molecular Biology (1966-67) and the Salk Institute for Biological

Studies (1967-69). In 1969 he was hired by James D. Watson to work

at the Cold Spring Harbor Laboratory in New York. Watson has been

reported to say this was the best hiring decision he ever made. Joe

was responsible for creating a combative creative environment at

CSHL that fomented discovery. Subsequently he worked at the

University of Texas Southwestern Medical Center (Dallas).

Sambrook is best known for his studies on DNA tumor viruses and

the molecular biology of normal and neoplastic cells. His Tumour

Virus Group at Cold Spring Harbor identified and mapped all of the

major genes of adenoviruses and SV40, determined their

transcriptional control in infected and transformed cells, and

elucidated the mechanism of integration of these viruses into the

genome of the host cell.[citation needed] He has also made

important contributions to the understanding of intracellular

traffic and protein folding and is an influential leader in the

field of the molecular genetics of human cancer.
目录:
VOLUME1
Part1：Essentials
1.IsolationandQuantificationofDNA
2.AnalysisofDNA
3.CloningandTransformationwithPlasmidVectors
4.GatewayRecombinationalCloning
JohnS.Reece-HoyesandAlberthaJ.M.Walhout
5.WorkingwithBacterialArtificialChromosomesandOtherHigh-CapacityVectors
NathanielHeintzandShiaochingGong
6.Extraction，Purification，andAnalysisofRNAfromEukaryoticCells
7.PolymeraseChainReaction
8.Bioinformatics
Jui-HungHungandZhipingWeng

VOLUME2
Part2：AnalysisandManipulationofDNAandRNA
9.QuantificationofDNAandRNAbyReal-TimePolymeraseChainReaction
10.NucleicAcidPlatformTechnologies
OliverRando
11.DNASequencing
ElaineMardisandW.RichardMcCombie
12.AnalysisofDNAMethylationinMammalianCells
PaulM.Lizardi，QinYan，andNarendraWajapeyee
13.PreparationofLabeledDNA，RNA，andOligonucleotideProbes
14.MethodsforInVitroMutagenesis
MatteoForloni，AlexLiu，andNarendraWajapeyee

Part3：IntroducingGenesintoCells
15.IntroducingGenesintoCulturedMammalianCells
PritiKumar，ArvindhanNagarajan，andPradeepD.Uchil
16.IntroducingGenesintoMammalianCells：ViralVectors
GuangpingGaoandMiguelSena-Esteves

VOLUME3
Part4：GeneExpression
17.AnalysisofGeneRegulationUsingReporterSystems
PradeepD.Uchil，ArvindhanNagarajan，andPritiKumar
18.RNAInterferenceandSmallRNAAnalysis
ChengjianLiandPhillipD.Zamore
19.ExpressingClonedGenesforProteinProduction，Purification，andAnalysis
ClaraL.Kielkopf，WilliamBauer，andInaUrbatsch

Part5：InteractionAnalysis
20.Cross-LinkingTechnologiesforAnalysisofChromatinStructureandFunction
TaeHoonKimandJobDekker
21.MappingofInVivoRNA-BindingSitesbyUV-Cross-LinkingImmunoprecipitation（CLIP）
JenniferC.Darnell，AldoMele，KaYingSharonHung，andRobertB.Darnell
22.Gateway-CompatibleYeastOne-HybridandTwo-HybridAssays
JohnS.Reece-HoyesandAlberthaJ.M.Walhout

Appendices
1.ReagentsandBuffers
2.CommonlyUsedTechniques
3.DetectionSystems
4.GeneralSafetyandHazardousMaterial

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9787030386069：分子克隆实验指南（第四版）

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